The enzyme, MTR94, was tested to determine its antimicrobial efficacy against a mixture of three common fungi and two common bacteria. Minimum Lethal Concentration (MLC) testing was the method used to determine the minimum concentration of enzyme required to kill the test microorganisms. MTR94 was found to be effective at killing the mixture of fungi and bacteria tested.
Procedure: All testing was performed in triplicate. The mean of the three results was considered the efficacy for that treatment. All microorganisms used were active cultures. For the first test, spores of Aspergillus "iger, Penicillin decumbens and Stachybotrys chartarum (three common species of fungi) were• mixed in sterile phosphate buffer to get a final concentration of 105 cfu/mL. Similarly, for the second and third tests, standard inoculums of E. coli and methicillin-resistant Staphylococcus aUfeUS (MRSA) were prepared to get a final concentration of 105 cfulmL. More...
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